Product Description
Distinguished scientists and researchers present a comprehensive collection of current preparative PCR techniques that can be used in cloning and modifying DNA and cDNA. Topics include performing and optimizing PCR (including long PCR), cloning PCR products, cloning unknown neighboring DNA, and library construction and screening. Also covered are mutagenesis, recombination, and in vitro selection, differential and subtractive approaches to cDNA analysis and screening, and cloning members of gene families. The techniques bring to both new and established researchers the power to apply PCR-based methodology to the cloning and modification of DNA, either through innovative protocols or by fostering individual creativity to modify and customize the protocols to best fit their own needs. ... Read more

Product Description
This three volume series represents a selected and refereedcollection of papers contributed by the participants of the FirstWorld Congress on Computational Medicine, Public Health, andBiotechnology, held in 1994 at Austin, Texas. Over 500 individuals,from 30 countries attended this meeting. In addition, this collectioncontains a number of papers from the Australian CSIRO High PerformanceComputing Meeting held that same year. ... Read more

Customer Reviews (3)

Nice try - but try again
This was required for my graduate level stats class.I honestly say I found it no use.Explainations were unclear, examples few, and it assumes you are more of a statisics major than biology.It has been sitting on my shelf for 6 years now and I get no use out of it.

Very Good Book
This was the main text for a Graduate Experimental Design class. I found the book very good especially for agriculture. It is not strictly a recipe book on how to do statistics. If you want that get the Gomez and Gomez book or Little and Hills. This book covers many topics well and has problem sets.

not useful for me
I bought the book because it was required in class. I just check it few times for some required exercises, but when the class was finished I sold the book almost new.
The fact is that I don't like the way they explain the topics, it's not very useful for me. I find better stuff on line than in that book. ... Read more

Product Description
As molecular and cellular biologists move toward nano techniques for performing experiments on single molecules rather than on populations of molecules, a comprehensive manual on how (and why) to carry out such experiments is needed. Single-Molecule Techniques: A Laboratory Manual fills this requirement--it is the first to take researchers who know nothing about single molecule analyses to the point where they can successfully design and execute appropriate experiments. Geared toward research scientists in structural and molecular biology, biochemistry, and biophysics, the manual will be useful to all who are interested in observing, manipulating, and elucidating the molecular mechanisms and discrete properties of macromolecules. Techniques range from in vivo and in vitro fluorescent based methods to the use of atomic force microscopy, optical and magnetic tweezers, and nanopores. The book is edited by Paul R. Selvin and Taekjip Ha, two pioneers in the field of experimental biophysics who have made significant contributions to the development and application of single molecule technologies. Related Titles from the Publisher Basic Methods in Microscopy; Gene Transfer: Delivery and Expression of DNA and RNA - A Laboratory Manual; Genomes; Molecular Cloning: A Laboratory Manual, Third Edition; The Condensed Protocols from Molecular Cloning: A Laboratory Manual; Protein-Protein Interaction: A Molecular Cloning Manual, Second Edition; Bioinformatics ... Read more

Product Description
This is a manual which aims to give students a chance to explore the process and techniques of characterizing and purifying a protein, and of subsequent cloning of the associated gene. Each chapter emphasizes the process of discovery, and the strategies and rationale for each experiment. To minimize cost and avoid extensive laboratory preparation, the experiments in this manual use readily available materials and organisms, the bacterium Escherichia coli and the yeast Saccharomyces carlsbergensis. Laboratory topics include: microbiological methods (handling, storage, media); organism growth and protein production; enzyme and protein essays (specific activity); batch purification of proteins; gel filtration and ion exchange chromatographies; genomic and plasmid DNA isolation; and DNA sequencing and computer analysis. ... Read more

Product Description
Cardiff Univ., UK. Features cutting-edge protocols for generating and maintaining genetically modified murine stains and includes techniques for cloning, conditional transgenesis, and cryopreservation of germlines. Emphasis is on manipulation of the mammalian genome. Previous edition: c1993. ... Read more

Customer Reviews (1)

Awesome!
This book offers a thrilling, roller-coaster ride from start to finish - I couldn't put it down!Essential!!!

JB ... Read more

Product Description
This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression.

Key Features
* Provides well-tested experimental protocols for each technique
* Lists the reagents and preparation of each experiment separately
* Contains a complete schedule of experiments and the preparation required
* Includes study questions at the end of each chapter ... Read more

Customer Reviews (1)

complete basic tool
Anyone whoneed very nice information should read it.The book provide quite day to day information to fit lab routine.It Also explainsome impresive insight on future technics. Wiht out doubt I recomended.I gave it5 star because I use it everyday at work. Lab.Gen e Molecular Biology.InCor. Brazil ... Read more

Product Description
The last few years have seen an upsurge of interest in the study of cells by optical microscopy. The advent of new techniques such as confocal microscopy and the availability of extremely sensitive digital imaging devices are revolutioniz-ing the field. A number of groups have developed new ways of making spectroscopic measurements at the microscopic level, accompanied by the introduction of appropriate sen-sor molecules for cellular assays. The aim of this volume will be to bring together the various advances in order to provide the reader with an up-to-date account of what can now be achieved with modern optical microscopic methods. ... Read more

Product Description
Cell culture techniques are invaluable to the modern researcher but difficult to carry out successfully. As part of the series of Handbooks in Practical Animal Cell Biology, this volume offers a concise practical guide to the basic essentials of the technique. Researchers new to cell culture will find a clear explanation of the essential equipment of a tissue culture facility, including tissue culture media and sera. It describes methods for growing suspension and adhesion cultures, including how to store cells and prepare primary cultures from cells.For those already culturing cells, the handbook will act as a handy reference to the basic techniques.The essence of the book is to deal with the generalities of cell culture and to give a grasp of the basic concepts before involvement in more specialized work in the field. Ideal for anyone moving into tissue cell culture techniques or looking for a concise reference book. ... Read more

Customer Reviews (2)

good content but.....
The content of this book is good, but come on, it is smaller than a typical novel - and not even printed/covered as nicely as a hard back novel - and it sells for $110.00 - buy the paperback it is actually printed and covered in higher quality at 1/3 the price.

From Book to Bench-Cell Culturing Made Easy
Whether a first year college student or a seasoned researcher, General Techniques of Cell Culture is a must.GTCC simplifies the complicated world of cell culturing without insulting. The authors, Maureen A. Harrisonand Ian F. Rae, make no assumptions about their readers, and take the taskof explaining large amounts of scientific information in an organized andeasy to read format. This handbook goes beyond general lab techniques andprovides not only the "how-to", but also gives the developing labthe where at, as it rounds out each section with a directory of servicesand researchable references. By providing time honored techniques, GeneralTechniques of Cell Culture also takes some of the trial and error out ofpropagating healthy cells in the lab. This alone should make the transitionfrom book to bench less tedius and more productive. ... Read more

Product Description
In this fifth edition of a highly popular text, undergraduate students are introduced to all the basic experimental techniques routinely used in practical biochemistry today. Most attention is given to techniques students will encounter in their practical classes, with the principles and theories behind them explained in detail to aid understanding. As a further aid to students, essential calculations and worked answers appear at the end of each chapter. "Key terms to understand" are also included to help students thoroughly review each topic. No contemporary book on modern biochemical techniques would be complete without chapters on molecular biology, recombinant DNA technology, genetic analysis and biomolecular interactions, and these topics have been extensively covered in this new edition. The book is essential reading for all bioscience undergraduate students and preclinical medical students for whom practical biochemistry, molecular biology and immunology form part of the syllabus. ... Read more

Customer Reviews (7)

Excellent Review
This is a very good review on the main techniques of biochemistry and molecular biology. Excellent for filling up those gaps that bad teachers left behind.

Tons of Info
It was more than I needed or could use. I tried to contact the seller to see if I could return it, but got no response.

Ok as an intro, but rather irksome
I had purchased this book as part of the required books for a molecular biology techniques class in college.While I did skim through most of it at that time, I hadn't given it my full attention.

Two years later, I've sat down and read the entire book (spare 2 chapters).My reason for doing this was to brush up on what I (hopefully) already knew, expand on my knowledge, and to catch some things I'd surely completely missed.

I do not like how the chapters are written by different authors.I suppose it makes sense in light of certain folks being experts in their respective fields... but isn't this book an intro book?Shouldn't the stuff in this book be "the basics"?I noticed that the style of writing (somewhat organization of thoughts, but mostly the tone/way of talking) varied.I also found more than one topic to be repeated in detail.Oh no, not just "refer to section ___ for more info"... nope... totally repeated.

I hate the organization of the book.I feel like the chapters are out of order.The chapters also, I feel, could have been focused differently to better tackle material.

Also, while a great deal of theory is helpful, I sure wished I'd seen some basic info included for actual benchwork.I wanted to see more of typical concentrations, troubleshooting, controls, etc.Because now I just look at this book and think, hmm... the info in that book I can get for free on the internet, probably faster and more detailed.I don't want to lug that thing around to work, etc.No way.Good for class though, I suppose.At least at the end of each chapter there is a recommendations section for further reading.

One of the best lab books available
I was blown away by this book.That's how good it is.

I'm a microbiology grad student working on my Ph.D. and I have referred to this book on multiple occasions.It covers almost every single type of procedure that a student would do in a life science or biochemistry laboratory.From molecular biology to PCR; from Southern blots to western blots; from electrophoresis to mass spectrometry; from cell culture to microscopy.....this book literally covers it all.Additionally, it covers all of these topics in pretty good detail.Each procedure is not only discussed in terms of how to do it, but also why it works.

Fantastic.Every life science, biochemistry, or health science student should have this as a reference book.

good with an adjunct
this is a very nice book with abundant information.there are few topics which are dealt extraordinarily especially centrifugation,HPLC,electrophoresis etcetera.i have been studying this book from my graduation.initially i used to feel some difficulty with this book,so i studied biopysicaland biophysical techniques by Upadhyaya and Upadhyaya.once i got the clear idea regarding the techniques,i shifted to this book.now i feel this book is very helpful.it is highly recommendedto allbiology students. ... Read more

Product Description

The landscape of immunocytochemistry has significantly evolved in past years. Once a laborious technique with limited application, the introduction of new detection systems and instrumentation has facilitated the widespread use of immunocytochemical methods. In the post-genomic era, the use of immunocytochemical methods has grown to include the identification of a broad spectrum of antigens including proteins, carbohydrates and lipids from virtually every organism. In Immunocytochemical Methods and Protocols, Third Edition, expert researchers explore the modern methods employed in the field today,  now widely used to identify, in situ, various components of cells and tissues in both normal and pathological conditions, and to demonstrate the localization of subcellular components. Chapters provide methods that are designed to be general in their application and to appeal to investigators across all disciplines, including those in research and clinical settings. Composed in the highly successful Methods in Molecular Biology™ series format, chapters contain a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

 Comprehensive and ground-breaking,  Immunocytochemical Methods and Protocols, Third Edition, brings together a variety of methods that are normally limited to applications in a specific area, providing a unique and vital guide for researchers and clinicians alike.

Product Description
Free radical species are generally short-lived due to their high reactivity and thus direct measurement and identification are often impossible. ESR is the only technique which has the potential for direct detection of radicals but in biological systems even these must be trapped by a spin-trapping agent. Thus most investigations involve recognition of indicators of the presence of radicals in vivo or "FOOTPRINTS" of radical-mediated damage.

Techniques in Free Radical Research assembles and critically assesses the most relevant and reliable experimental approaches used towards the measurement of radicals and radical-mediated damage in chemical systems, in cells and in tissues under the following six headings: a) Footprints of DNA damage, b) Footprints of protein damage, c) Footprints of lipid peroxidation, d) Footprints of antioxidant consumption, e) Footprints via indirect radical assays, and f) Footprints via the availability of transition metal complexes.
... Read more

Product Description
The techniques described in this book provide an alternative method to understanding cellular biological structures and processes. Molecular genetic methods mainly analyse single molecules or very small cellular parts, which does not necessarily enhance our understanding of the fundamental cellular processes. However, using techniques such as atomic force or infrared microscopy, neutron reflection, stopped-flow cytometry, laser microscopy, or biosensoric-based electronics, it is possible to study cells and biological systems in their entirety. Physiological processes of cells, such as movement, development, plasticity, regeneration and communication, can be visualized using the high precision biophysical techniques described here. ... Read more