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In situ hybridization is a technique that allows for the visualization of specific DNA and RNA sequences in individual cells, and is an especially important method for studying nucleic acids in heterogeneous cell populations. in situ Hybridization in Electron Microscopy reviews the three main methods developed for the ultrastructural visualization of genes: °hybridization on ultrathin sections of tissue embedded in hydrophilic resin (post-embedding method)°hybridization prior to embedding (pre-embedding)°hybridization on ultrathin sections of frozen tissue (frozen tissue method).For each technique, the different stages are described in detail: the preparation of tissue, pretreatment, hybridization, and visualization of the hybridization products.The book combines theory and practice, starting with the basic principles, then breaking down the experimental process into successive steps illustrated by numerous diagrams, detailed protocols, and tables. This is all done in a format that uses parallel columns to convey useful comments next to the theory and practical details alongside each stage of the protocol. Additionally, the summary tables provide the criteria for choosing the probe type and technique, and a detailed index aids in the search for information. in situ Hybridization In Electron Microscopy is an essential companion for applying these methods at the electron microscopic level. ... Read more

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This book provides the reader with a comprehensive introduction to high-energy electron diffraction and elastic and in elastic scattering of high-energy electrons, with particular emphasis on application to modern electron microscopy. Starting from a survey of fundamental phenomena, we introduce the most important concepts underlying modern understanding of high-energy electron diffraction. Dynamical diffraction in transmission (THEED) and reflection (RHEED) geometries is treated using a general matrix theory, where computer programs and worked examples are provided to illustrate the concepts and to familiarize the reader with practical applications.Diffuse and inelastic scattering and coherence effects are treated comprehensively both as a perturbation of elastic scattering and within the general multiple scattering quantum mechanical framework or the density matrix method.Among the highlights are the treatment of resonance diffraction of electrons, HOLZ diffraction, the formation of Kikuchi bands and lines and ring patterns, and application of diffraction to monitoring of growing surfaces.Useful practical data are summarized in tables including those of electron scattering factors for all neutral atoms and many ions, and the temperature dependent Debye-Waller factors given for over 100 elemental crystals and compounds. ... Read more

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This book contains all the necessary information and advice for anyone wishing to obtain electron micrographs showing the most accurate ultrastructural detail in thin sections of any type of biological specimen.

The guidelines for the choice of preparative methods are based on an extensive survey of current laboratory practice. For the first time, in a textbook of this kind, the molecular events occurring during fixation and embedding are analysed in detail. The reasons for choosing particular specimen preparation methods are explained and guidance is given on how to modify established techniques to suit individual requirements.

All the practical methods advocated are clearly described, with accompanying tables and the results obtainable are illustrated with many electron micrographs.

Portland Press Series: Practical Methods in Electron Microscopy, Volume 17, Audrey M. Glauert, Editor ... Read more

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Must have reference for specimen preparation
Excellent reference book, a true must have if you need to embed samples for electron microscopy. The author very thoroughly explains the steps for sample preparation and the embedding media available. ... Read more

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This is a clear and up-to-date account of the application of electron-based microscopies to the study of high-Tc superconductors. Written by leading experts, this compilation provides a comprehensive review of scanning electron microscopy, transmission electron microscopy and scanning transmission electron microscopy, together with details of each technique and its applications. Introductory chapters cover the basics of high-resolution transmission electron microscopy, including a chapter devoted to specimen preparation techniques and microanalysis by scanning transmission electron microscopy. Ensuing chapters examine identification of new superconducting compounds, imaging of superconducting properties by low-temperature scanning electron microscopy, imaging of vortices by electron holography and electronic structure determination by electron energy loss spectroscopy. The use of scanning tunneling microscopy for exploring surface morphology, growth processes and the mapping of superconducting carrier distributions is also discussed. Final chapters consider applications of electron microscopy to the analysis of grain boundaries, thin films and device structures. Detailed references are included. This book will interest graduate students and researchers in condensed matter physics and material science. ... Read more

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North Carolina State University, Raleigh. Student textbook intended for a one-semester course on the use of the transmission and scanning electron microscopes. Includes specimen preparation, staining, cryotechniques, and more. Half-tone illustrations. ... Read more

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Harvard Medical School, Boston, MA. Atlas presents the basic diagnostic criteria used by the electron microscopist in studying neoplasms and other diseases encountered in the routine practice of pathology. Includes full-page, halftone plates. Includes more than 894 first-quality images. Previous edition: c1988. DNLM: Microscopy Electron--methods Atlases. ... Read more

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The discovery of the Nanotube in 1991 by electron microscopy has ushered in the era of Nanoscience. The atomic-resolution electron microscope has been a crucial tool in this effort. This book gives the basic theoretical background needed to understand how electron microscopes allow us to see atoms, together with highly practical advice for electron microscope operators. The book covers the usefulness of seeing atoms in the semiconductor industry, in materials science (where scientists strive to make new lighter,stronger, cheaper materials), and condensed matter physics (for example in the study of the new superconductors). Biologists have recently used the atomic-resolution electron microscope to obtain three-dimensional images of the Ribosome, work which is covered in this book. The books also shows how the ability to see atomic arrangements has helped us understand the properties of matter.

This new third edition of the standard text retains the early section of the fundamentals of electron optics, linear imaging theory with partial coherence and multiple-scattering theory. Also preserved are updated earlier sections on practical methods, with detailed step-by-step accounts of the procedures needed to obtain the highest quality images of the arrangement of atoms in thin crystals using a modern electron microscope. The sections on applications of atomic resolution transmission electron microscopy (HREM) have been extensively updated, including descriptions of HREM in the semiconductor industry, superconductor research, solid state chemistry and nanoscience, as well as metallurgy, mineralogy, condensed matter physics, materials science and biology. Entirely new sections have been added on electron holography, aberration correctors, field-emission guns, imaging filters, HREM in biology an don organic crystals,super-resolution methods, Ptychography, CCD cameras and Image plates. New chapters are devoted entirely to scanning transmission electron microscopy and Z-contrast, and also to associated techniques, such as energy-loss spectrocospy, Alchemi, nanodiffraction and cathodoluminescence. Sources of software for image interpretation and electron-optical design are also given. ... Read more

Customer Reviews (1)

A classic take on high-resolution transmission electron microscopy
I bought the original 1980 addition 25 five years ago as a post-doc looking to get into high-resolution transmission electron microscopy (HRTEM).This book gave me what I needed to get started and publishing in what was then a fairly new field.Now imaging atomic lattices is fairly commonplace and HRTEM has gone well beyond the what was state-of-the-art in 1980.Nevertheless, if any topic is ever explained clearly even once, nobody will every bother again, so this is still a useful book in many ways.I found the introduction to phase contrast microscopy at the beginning of the book especially valuable--particularly in its use of actual electron micrographs to illustrate the appearance of a bright fringe at the edge of an object for the under focus condition, with a dark finge visible in the over focus condition.When read along with Cowley's book Diffraction Physics the reader has a pretty good picture of the still very useful conventional HRTEM technique. ... Read more

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This volume is produced from digital images created through the University of Michigan University Library's large-scale digitization efforts. The Library seeks to preserve the intellectual content of items in a manner that facilitates and promotes a variety of uses. The digital reformatting process results in an electronic version of the original text that can be both accessed online and used to create new print copies. The Library also understands and values the usefulness of print and makes reprints available to the public whenever possible. This book and hundreds of thousands of others can be found in the HathiTrust, an archive of the digitized collections of many great research libraries. For access to the University of Michigan Library's digital collections, please see http://www.lib.umich.edu and for information about the HathiTrust, please visit http://www.hathitrust.org ... Read more